The clinic value of peripheral blood AFP mRNA and ALB mRNA in diagnosis and monitoring metastasis of hepatocellular carcinoma / 中国医师杂志

ABSTRACT

Objective To explore the clinical value of peripheral blood albumin gene and alpha-fetoprotein gene in early diagnosis and differential diagnosis ofhepatocellular carcinoma(HCC).Methods Two gene fragments were amplified by nested reverse transcription polymerase chain reaction(RT-PCR)with the samples from patients.Results The positive tales of peripheral blood gene fragments from patients with HCC were 53.7％in AFP and 59.7％in Alb.respectively.The former Wag signifieantly higher than that in liver cirrhosis(LC),chronic hepatitis(CH),acute hepatitis(AH)and extrahepatic tumor(ET).The latter Wag also significantly higher than that in LC,CH and ET(P<O.01),but there were no remarkably difference in the positive rate of Alb.mRNA between HCC and AH.The presenee of AFP-mRNA and AIb-mRNA were correlmed with the stage of HCC and intrahepatic metastasis.Conclusion The results suggest that gene fragments of peripheral blood AFP and Alb are useful marker8 for diagnosis of HCC,differentiating extrahepatic metastasis of HCC and monitoring recurrence after HCC operation.