Effects of focal mild hypothermia on inflammation after traumatic brain injury in rats / 中华急诊医学杂志

ABSTRACT

Objective To study the effects and mechanism of focal mild hypothennia on brain inflarnmation response during the early stage of tranrntic brain injury(TBI)in rats.Method Traumatic brain injury wag produced in anesthetized rats by using free-falling impact model.Sixty-three male Sprague Dawley rats were randomly divided into three groupsTBI group,focal hypothermia group,and sham group.each group with 7 SD rats.Then all groups Wag further subdivided into three subgroups according to different intervals after TBI.Myeloperoxiduse (MPO)activity assay,immunohistochemical staining for the detennination of intercellular adhesion molecule-1(ICAM-1)expression and the number of ICAM-1 positive blood vessels were counted up in the traumatic brain tissues.All data were analyzed by SNK-q test Using SPSS 10.0 sofeware program.Results The number of ICAM1 positive blood vessels was significantly fewer in the rats of focal hypothermia group than those in the rats of TBI group at different intervals after TBI(P<0.01).Similarly,the MPO activity was significantly lower in the rats of focal hypothermia group than that in the rats of TBI group(P<0.01).Conclusions Focal mild hypothermia could significantly decrease neutrophil infdtration and ICAM-1 expression in the traumatized areas.It might be one of the mechanisms of focal mild hypothermis for TBI.