Production and Characterization of Monoclonal Antibodies to Mumps Virus Isolated in Korea
Journal of Bacteriology and Virology
;
: 203-208, 2003.
Article
Dans Coréen
| WPRIM
| ID: wpr-39997
ABSTRACT
Classical mumps patients develop bilateral or less commonly unilateral parotitis. Mumps virus belongs to the genus Rubulavirus in the family Paramyxoviridae. It contains single stranded RNA genome with negative polarity. To characterize the antigenicity of mumps virus isolated in Korea, nineteen hybridoma cell lines producing monoclonal antibodies to mumps virus were established by fusion of Sp2/0-Ag14 mouse myeloma cells with spleen cells of mice immunized with mumps virus strain 98-40. The specificity of these monoclonal antibodies was established by immunofluorescence microscopy and immunoblotting analysis. Fifteen out of nineteen hybridoma cell lines secreted IgG monoclonal antibodies (MAbs) against mumps virus, and the remaining four secreted IgM. The isotypes of thirteen clones of 19 MAbs were IgG1, two were IgG2a, and four were IgM. Eight MAbs reacted with a 68 kDa nucleocapsid protein, six MAbs reacted with a 46 kDa phosphoprotein, and five MAbs reacted with a 42 kDa matrix protein.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Parotidite
/
Rate
/
Paramyxoviridae
/
Immunoglobuline G
/
Immunoglobuline M
/
ARN
/
Immunotransfert
/
Lignée cellulaire
/
Sensibilité et spécificité
/
Génome
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Asie
langue:
Coréen
Texte intégral:
Journal of Bacteriology and Virology
Année:
2003
Type:
Article
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