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Influence of various volume fractions of platelet-rich plasma on dog bone marrow mesenchymal stem cell proliferation / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 9727-9730, 2009.
Article Dans Chinois | WPRIM | ID: wpr-404667
ABSTRACT

BACKGROUND:

Proliferation and differentiation of mesenchymal stem cells (MSCs) is associated with platelet concentration in platelet-rich plasma (PRP). Low enrich multiple cannot reach proper effects, but high level had inhibitory effects on osteoanagenesis.

OBJECTIVE:

To observe the effect of different volume fraction of PRP on dog BMSC proliferation.

DESIGN:

A cytological in vitro study.MATERIALS Healthy 12-month male Beagle dogs were supplied by the Experimental Animal Center, Xiangya Medical College,Central South University.

METHODS:

Dog BMSCs of 5 passage were adjusted to 3×10~8/L, and incubated in a 96-well plate at 200 μL per well. Following 24 hours of routine culture, primary medium and non-adherent cells were discarded. Prepared PRP gel was mixed with serum-free low-glucose DMEM containing penicillin and streptomycin, and then diluted into 5%, 6.25%, 7.5%, 8.75%, 10% volume fraction. 200 μL above-described liquid was added into the 96-well plate, which was subsequently placed in a incubator.We set up a blank control.MAIN OUTCOME

MEASURES:

MTT was used to investigate effect of different volume fraction of PRP on dog BMSC proliferation.

RESULTS:

Compared with the blank control group, various volume fraction of PLP could promote dog BMSC proliferation in early stage. With prolonged time, proliferation speed began to increase at day 6 in the 8.75% and 10% PRP groups, entering platform stage. BMSC number was increased rapidly in the 5% and 6.25% PRP groups, especially in the 6.25% PRP group.

CONCLUSION:

PRP gel could promote BMSC proliferation markedly and proliferation strength of BMSCs was correlated to the density of PRP. BMSC proliferation would be accelerated by the low density of PRP.
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) langue: Chinois Texte intégral: Chinese Journal of Tissue Engineering Research Année: 2009 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) langue: Chinois Texte intégral: Chinese Journal of Tissue Engineering Research Année: 2009 Type: Article