Effects of intravascular low dose He-Ne laser irradiation on rats after ischemia reperfusion

Li HE; Zhijian ZHANG; Shuangyan KONG; Hongbo ZHENG

ABSTRACT

BACKGROUND:

Quite a number of in vitro experiments indicate that low dose He-Ne laser can stimulate the cell growth and blood vessel regeneration therefore to improve the damages caused by ischemia. However, it needs further study on the in vivo experiments.

OBJECTIVE:

To study the protective effects of intravascular low dose laser irradiation to ischemic reperfusion of rats and explore its mechanism.

DESIGN:

A randomised and controlled trial based on experinental animals.

SETTING:

Department of Neurology of a hospital of a university.MATERIALS The experiment was completed in the Surgery Experimental Animal Center of West China Hospital of Sichuan University. Healthy male SD rats of class Ⅱ which aged between 5 to 6 months with weight of 372 to 418 grams[mean weight(388.48 ± 10. 57) g] were provided by Animal Research Center of Sichuan University.

INTERVENTIONS:

Middle cerebral artery ischemia model of rats was prepared. Forty SD rats were randomly divided into treatment group and control group with each of 20 rats. Intravascular low dose He-Ne laser irradiation was conducted to rats in treatment group one time every other day, 20 minutes per time and 3 times for a course. Vein puncture was conducted to rats in control group without laser input.MAIN OUTCOME

MEASURES:

Ratio of infarcted volume

RESULTS:

The ratio of the cerebral infarction volume in treatment group was (10.43 ± 1.04) and is much lower than that of control group(16.78 ± 1.12).There was statistical difference between two groups( t = 27.14, P ＜ 0.05).

CONCLUSION:

Intravascular low dose He-Ne laser irradiation has protective effects to cerebral ischemia of rats.