Cloning, Expression and Activity of Recombinant Human Angiostatin / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6): 285-287, 2000.
Article
Dans Chinois
| WPRIM
| ID: wpr-412395
ABSTRACT
Objective:
To express recombinant human angiostatin for further application in clinic.Methods:
The complete encoding eDNA of human angiostatin was isolated from human embryo liver with RT-PCR and expressed in secretory Pichia expression system. Recombinant human angiostatin was purified with heparin sepharose chromatography and its activity was determined in chick embryo chorioallantoic membrane (CAM) and wound healing assays.Results:
Expressed in large quantity (yield=5 mg/L) and purified with heparin sepharose, recombinant angiostatin was showed to have a molecular weight of 43 kD in SDS-PAGE and potently inhibit angiogenesis and wound healing.Conclusion:
Recombinant human angiostatin was expressed efficiently in a biologically active form.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Cancer Biotherapy
Année:
2000
Type:
Article
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