Growth inhibition and apoptosis induced by oridonin combined with valproic acid in HL-60 cells / 国际肿瘤学杂志
Journal of International Oncology
; (12): 390-393, 2011.
Article
de Zh
| WPRIM
| ID: wpr-415401
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WPRO
ABSTRACT
Objective To investigate the apoptosis-inducing effect of oridonin combined with valproic acid( VPA)on leukemic cell line HL-60,and study the feasibility of oridonin combined with VPA to be used in clinical practice. Methods Oridonin of 6-12 μmol/L combined with VPA of 0. 5-1 mmol/L were added in exponential growth HL-60 cells respectively. Cell count assays were used to measure the growth inhibitory effect of oridonin combined with VPA or alone. Flow cytometry was used to evaluate apoptosis with Annexin V and propidium iodide (PI) double staining. Results Combined use of oridonin and VPA could synergistically inactivate HL-60 cells,and inhibit the cell proliferation and induce apoptosis in a dose-and time-dependent manner (P < 0.05 ). Conclusion Oridonin has a synergistic effect combined with VPA. Oridonin has a promising prospect in clinical use of leukemia.
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WPRIM
langue:
Zh
Texte intégral:
Journal of International Oncology
Année:
2011
Type:
Article