Determination of Nuezhenide and Apigenin 7-O-neohesperidoside in Shanxiangyuan tablets by HPLC / 国际中医中药杂志

ABSTRACT

ObjectiveTo establish a method for the determination of Nuezhenide and Apiganin 7-O-neohesperidoside in Shanxiangyuan tablets.Methods The Luna C1s column was used,the mobile phase consisted of HCN-0.5％H3PO4(41 ∶ 59),the flow rate was 1.0 ml/min,the column temperature was 30℃,the UV detector was set at 336nm.Results The linear response range was 0.016～0.32 mg/ml (r=0.9997) and 0.006～0.11 mg/ml respectively.The average recovery rate was 99.0％ and 97.5％ respectively,RSD was 1.83％ and 1.57％ respectively,ConclusionThe method was simple,accurate and repeatable.It can be applied in quantitative determination of Nuezhenide and Apigenin 7-O-neohesperidoside in Shanxiangyuan tablets.