Apoptosis of hypertrophic scar fibroblasts induced by HMME-PDT / 中华医学美学美容杂志
Chinese Journal of Medical Aesthetics and Cosmetology
;
(6): 381-384, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-419853
ABSTRACT
Objective To investigate the apoptotic effects of hypertrophic scar fibroblast (HSF) induced by HMME-PDT.Methods Fibroblasts were cultured from nontreated hypertrophic scars,and cells at passages 4-6 were used for the experiments (photosensitizer dose 4 μg/ml,λ630 nm,pow er density 10 mw/cm2,energy fluence 2.5 J/cm2).Morphological and biochemical changes in fibroblasts were assessed by Hoechst 33258 staining and fluorescence microscopy.The rate of apoptotic or necrotic cells was detected by flow cytometry (FCM) through double staining of Annexin V -FITC and popodium iodide (PI),respectively.Results Marked morphological features of cell apoptosis were viewed under the fluorescent microscope through Hoechst 33258 staining.The analysis of FCM indica ted that the apoptotic rate was significantly increased after HMME PDT [(34.82 ± I.42) % vs (3.12±0.28) %,P<0.05],and apoptotic rate was higher than necrosis rate [(14.65±1.02) % vs (34.82±1.42) %,P<0.05].Conclusions Low level exposure to 630 nm PDT mediated by HMME appears to induce fibroblast apoptosis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Aesthetics and Cosmetology
Année:
2011
Type:
Article
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