Characterization of human herpesvirus-8 genotypes in squamous cell carcinoma by using the polymorphisms in open reading frame 26 gene / 中华皮肤科杂志

ABSTRACT

Objective To analyze the genotypes of human herpesvirus 8 (HHV-8) by using the polymorphisms in open reading frame(ORF) 26 gene in patients with squamous cell carcinoma (SCC)in Xinjiang Uygur Autonomous Region.MethodsDNA was extracted from paraffin-embeded tissue specimens from 41 patients with skin SCC and 46 patients with esophagus SCC,and subjected to nested-PCR for the amplification of the ORF26 gene of HHV-8 followed by bidirectional sequencing.Phylogenetic analysis was carried out to determine the genotype of HHV-8 by using the DNASTAR software,Clustal W program,and PHYLIP package.The data were analyzed by SPSS 17.0 software.ResultsHHV-8 DNA was detected in 9 (21.95％) of 41 skin SCC specimens and 10 (21.74％) of 46 esophagus SCC specimens (x2 =0.09,P＞ 0.05).As phylogenetic analysis showed,7 HHV-8 isolates from skin SCC specimens belonged to ORF26 subtype A,2 subtype C; 7 HHV-8 isolates from esophagus SCC specimens belonged to ORF26 subtype A,and 3 subtype C.Conclusions ORF26 subtype A and C are the predominate genotypes of HHV in patients with SCC in Xinjiang Uygur Autonomous Region,with the prevalence of subtype A higher than that of subtype C.The distribution of HHV subtypes seems unrelated to the location of SCC.