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Effect of electromagnetic radiation at different bands on the expressions of SOX9 and WT1 in rat Sertoli cells / 中华放射医学与防护杂志
Chinese Journal of Radiological Medicine and Protection ; (12): 47-51, 2012.
Article Dans Chinois | WPRIM | ID: wpr-424837
ABSTRACT
Objective To explore the changes of SOX9 and WT1 expressions in rat Sertoli cells irradiated by EMP ( electromagnetic pulse),S-HPM ( S-band high power microwave) and X-HPM ( Xband high power microwave).Methods Primary Sertoli cells were isolated from 3-week-old Wistar rats and its purity was immunocytochemistrically indentified with WT1.After exposure to 6 × 104 V/m EMP,100 mW/cm2 S-HPM and X-HPM for 4 min respectively,SOX9 and WT1 expressions in Sertoli cells were determined with real-time PCR and Western blot,respectively.Results SOX9 mRNA expression was decreased at 6 and 12 h post-irradiation of three different bands of electromagnetic microwave ( F =15.20and 4.84,P < 0.05 ).SOX9 protein expression was also decreased at 6 and 24 h after irradiation ( F =8.46 and 7.47,P<0.05).WT1 mRNA expression was decreased at6 and 12 h (F=13.46 and 5.08,P < 0.05 ),but its protein expression was decreased only at 24 h post-irradiation ( F =10.26,P < 0.05 ).Conclusions Three bands of electromagnetic radiation reduce the expressions of SOX9 and WT1 in rat Sertoli cells,which may provide molecular foundation for genital system hazards induced by microwave radiation.

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) langue: Chinois Texte intégral: Chinese Journal of Radiological Medicine and Protection Année: 2012 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) langue: Chinois Texte intégral: Chinese Journal of Radiological Medicine and Protection Année: 2012 Type: Article