Your browser doesn't support javascript.
loading
Comparison of EGF with VEGF Non-Viral Gene Therapy for Cutaneous Wound Healing of Streptozotocin Diabetic Mice
Diabetes & Metabolism Journal ; : 226-235, 2011.
Article Dans Anglais | WPRIM | ID: wpr-42486
ABSTRACT

BACKGROUND:

To accelerate the healing of diabetic wounds, various kinds of growth factors have been employed. It is the short half-life of administered growth factors in hostile wound beds that have limited wide-spread clinical usage. To overcome this limitation, growth factor gene therapy could be an attractive alternative rather than direct application of factors onto the wound beds. We administered two growth factor DNAs, epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) into a cutaneous wound on diabetic mice. We compared the different characteristics of the healing wounds.

METHODS:

Streptozotocin was injected intraperitoneally to induce diabetes into C57BL/6J mice. The ultrasound micro-bubble destruction method with SonoVue as a bubbling agent was used for non-viral gene delivery of EGF828 and VEGF165 DNAs. Each gene was modified for increasing efficacy as FRM-EGF828 or minicircle VEGF165. The degree of neoangiogenesis was assessed using qualitative laser Doppler flowmetry. We compared wound size and histological findings of the skin wounds in each group.

RESULTS:

In both groups, accelerated wound closure was observed in the mice receiving gene therapy compared with non treated diabetic control mice. Blood flow detected by laser doppler flowmetry was better in the VEGF group than in the EGF group. Wound healing rates and histological findings were more accelerated in the EGF gene therapy group than the VEGF group, but were not statistically significant.

CONCLUSION:

Both non-viral EGF and VEGF gene therapy administrations could improve the speed and quality of skin wound healing. However, the detailed histological characteristics of the healing wounds were different.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phospholipides / Peau / Hexafluorure de soufre / Cicatrisation de plaie / ADN / Thérapie génétique / Fluxmétrie laser Doppler / Streptozocine / Protéines et peptides de signalisation intercellulaire / Facteur de croissance endothéliale vasculaire de type A Type d'étude: Recherche qualitative Limites du sujet: Animaux langue: Anglais Texte intégral: Diabetes & Metabolism Journal Année: 2011 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phospholipides / Peau / Hexafluorure de soufre / Cicatrisation de plaie / ADN / Thérapie génétique / Fluxmétrie laser Doppler / Streptozocine / Protéines et peptides de signalisation intercellulaire / Facteur de croissance endothéliale vasculaire de type A Type d'étude: Recherche qualitative Limites du sujet: Animaux langue: Anglais Texte intégral: Diabetes & Metabolism Journal Année: 2011 Type: Article