Expression of stomatin like protein-2 in gastric adenocarcinoma and its effects on the migration and invasion of SGC7901 cell / 中华消化杂志

ABSTRACT

Objective To investigate the expression of stomatin like protein 2 (SLP-2) in gastric adenocarcinoma and its effects on migration and invasion of SGC7901 cell line.Methods The expression of SLP-2 in 60 pairs of gastric adenocarcinoma tissue specimens and corresponding adjacent tumor tissue specimens was detected by immunohistochemistry,and its relation with clinical pathological characteristics was also analyzed.SGC7901 cells were transient transfected with SLP-2 siRNA.After transfection,the expression of SLP-2 in SGC7901 cells at mRNA and protein level was detected by reverse transcripton-polynerase chain reaction (RT-PCR) and Western blot.The changes of migration and invasion of SGC7901 cells after transfection were observed through Transwell test and Matrigel in vitro invasion test.The data were analyzed by x2 test and LSD-t test.Results The high expression percentage of SLP-2 in gastric adenocarcinoma tissue was 68.3％ (41/60),which was significantly higher than that of the adjacent tumor tissues (25.0％,15/60; x2 =22.634,P＜0.01).The expression of SLP-2 in late stage gastric adenocarcinoma tissues was higher than that of early stage gastric adenocarcinoma tissues (x2 =4.962,P＜0.05).The expression of SLP-2 in gastric adenocarcinoma tissues with lymph nodes metastasis was higher than that in gastric adenocarcinoma tissues without lymph nodes metastasis (x2 =3.994,P＜0.05).There was no correlation between the expression and gender,age,tumor size and differentiation degree (all P＞0.05).After SLP-2 siRNA transfection,the expression of SLP-2 in SGC7901 cells significantly decreased at both mRNA and protein level.At 48 h after SLP-2 siRNA transfection,the cell migration ability was significantly lower (migrated cell number 53.0± 10.8) than that of negative control group (75.8±13.4) and blank control group (78.4± 15.2) (t=22.778 and 25.444,both P＜0.01).The in vitro invasion ability of SGC7901 cells (migrated cell number 36.3 ± 8.9) was also significantly lower than that of negative control group (53.9±12.1) and blank control group (49.8±9.0) (t=17.556 and 13.444,both P＜0.01).Conclusions The expression of SLP-2 was high in gastric adenocarcinoma tissues.SLP-2 may involve in the metastasis of gastric adenocarcinoma through promoting the migration and invasion ability of gastric adenocarcinoma cell.