PLOD2 is involved in human skin fibrosis / 基础医学与临床

ABSTRACT

Objective To discuss the relationship between the expression of PLOD2 and the formation of collagen I in the scar. Methods To detect the mRNA expression of PLOD2 and COLIα1 in the scar and normal skin tis-sue, as well as the content of COLIα1 by RT-PCR and Western blot; design and synthesize three kinds of PL0D2 ASODN targeted at the translational initiation, the joint of the first and the second exons and termination codon re-spectively , transfected into NIH/3T3 mouse fibroblast cell and detect the mRNA expression of COLIα1 and forma-tion of collagen I by RT-PCR and Western blot. Results The expression of PLOD2 in the scar tissue was signifi-cantly higher than that in the normal tissue ( P < 0. 05 ) , three kinds of ASODNs showed inhibitory effects on the mRNA expression of COLIα1 and formation of collagen I in fibroblast (P <0. 05) , during which the repressive ef-ficiency of ASODN targeted against the translational initiation was the highest (P <0. 001) ; while the mRNA ex-pression level of COLIα1 did not change in the scar tissue, normal tissue and the groups treated with three kinds of ASODN. Conclusion The high expression of PLOD2 is tightly related to the formation of collagen I , PLOD2 ASODN can suppress the formation of collagen I post-transcriptionally.