Neurogenin2 gene-regulated Schwann cells differentiate into neurons / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 8590-8595, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-440946
ABSTRACT
BACKGROUND:
It is confirmed that astrocytes can differentiate into neurons by Neurogenin2 gene regulation, suggesting that Schwann cells may also differentiate into neurons by gene regulation.OBJECTIVE:
To evaluate the feasibility of Schwann cells differentiating into neurons by Neurogenin2 gene regulation.METHODS:
Rats Schwann cells were isolated, purified and identified. Then the Schwann cells were transfected with Neurogenin2 via green fluorescent protein gene-plentivirus. To induce neuronal differentiation, the Schwann cells were cultured in serum-free Dulbecco’s modified Eagle’s medium containing epidermal growth factor, basic fibroblast growth factor and brain-derived neurotrophic factor for 2 weeks. The morphology of induced cells was observed by microscope, and myelin basic protein and neuron-specific enolase were detected by immunocytochemistry. RESULTS ANDCONCLUSION:
After transfection with Neurogenin2 via green fluorescent protein gene-plentivirus and induced differentiation, immunofluorescence assay demonstrated that 12.56%of the induced cellexpressed neuron-specific enolase, but the control group did not express neuron-specific enolase. Neurogenin2 gene-transfected Schwann cells can express neuron-specific enolase, suggesting Neurogenin2 gene may regulate transdifferentiation of Schwann cells into neurons.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Chinese Journal of Tissue Engineering Research
Année:
2013
Type:
Article
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