Determination of Gallic Acid in 18 Taste Myrobalan Diuretic Pill by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish the method for determination of gallic acid in Shibawei Heziliniao Pill by HPLC. Methods ODS-2 HYPERSIL C18 column (250 mm×4.6 mm, 5 μm) was used, with mobile phase of methanol-0.1%phosphoric acid solution (7∶93). The flow rate was 1.0 mL/min, the detection wavelength was 270 nm, and the column temperature was 30 ℃. Results Gallic acid showed a good linear relationship within the range of 0.063-0.315 μg, Y=2902.1X-5.95, r=0.999 6. The average recovery was 98.56%, and RSD was 0.41% (n=5). Conclusion The method is sensitive, simple, accurate, reproducible, and can be used for quality control of 18 Taste Myrobalan Diuretic Pill.