Effects of basic fibroblast growth factor gene transfection on the proliferation of umbilical cord mesenchymal stem cells / 中国组织工程研究

ABSTRACT

BACKGROUND:At present, exogenous basic fibroblast growth factor gene can be transfected into umbilical cord mesenchymal stem cells via a recombinant adeno-associated virus vector and exhibit sustained expression in transfected cells. This method can regulate cellproliferation and directed differentiation to obtain efficient long-lasting therapeutic effects. OBJECTIVE:To investigate the effects of basic fibroblast growth factor gene transfection via a recombinant adeno-associated virus vector on the proliferation and cellcycle of human umbilical cord mesenchymal stem cells cultured in vitro. METHODS:Human umbilical cord mesenchymal stem cells were cultured by the suspension culture in vitro, and were transfected by recombinant adeno-associated virus-mediated basic fibroblast growth factor gene. Cultured cells were divided into three groups:control group, basic fibroblast growth factor group, and recombinant adeno-associated virus group. Reverse transcription-PCR and western blot were used to assess the knockdown efficiency. cellular proliferation was determined by cellgrowth curve and cellCounting Kit-8 assay. The cellcycle was analyzed by flow cytometry. RESULTS AND CONCLUSION:Compared with the other two groups, the expression of basic fibroblast growth factor mRNA and protein increased significantly, the cellgrowth speed was also significantly increased, the cellcycle of G0/G1 phase was decreased and cellnumber in S phase was increased in the basic fibroblast growth factor group after transfection. These findings suggest that the recombinant adeno-associated virus-mediated basic fibroblast growth factor gene can promote the proliferation of umbilical cord mesenchymal stem cells proliferation cultured in vitro, and also can optimize the cellculture.