Application value of combined detection of AFP-L3,GP73 and GPC3 in diagnosing primary hepatic cancer / 国际检验医学杂志

ABSTRACT

Objective To study the clinical value of the combination detection of serum tumor markers alpha fetal protein hetero-geneity-L3(AFP-L3) ,Golgi protein 73(GP73) and phosphatidylinositol proteoglycan-3(GPC-3) in the diagnosis of primary hepatic cancer .Methods The serum AFP-L3 ,GP73 and GPC-3 levels were measured in 34 patients with primary hepatic cancer (PHC) ,20 patients with liver cirrhosis ,20 patients with chronic hepatitis B ,37 patients with other tumors by the enzyme linked immunosor-bent assay(ELISA) .Meanwhile ,20 individuals with healthy physical examination were selected as the control group .The expression situation of various indexes were compared and analyzed .Results The expression levels of AFP-L3 ,GP73 and GPC-3 in the PHC group were significantly higher than those in the control group and the other tumors groups ,difference was statistically significant (P<0 .05) .The areas under the receiver operating characteristic (ROC) curve of PHC in the single item detection of 3 markers were 0 .909 ,0 .832 and 0 .817 respectively .The areas of the ROC curve in the combination examinations of two items were 0 .935 , 0 .945 and 0 .912 respectively .The area of the ROC curve in the 3-item combined detection of PHC could be up to 0 .960 .Conclusion The combined detection of AFP-L3 ,GP73 and GPC-3 can increase the detective rate of PHC and has important clinical significance to early diagnosis of PHC .