Intervention effects of SOD-enriched cili juice on immune function in arseniasis rats caused by coal burning / 中国药理学与毒理学杂志

ABSTRACT

OBJECTIVE To explore the influence of superoxidase (SOD)-enriched cili juice (CJSOD)on immune function of arseniasis rats,in order to provide a experi mental basis for the search of drugs of immune regulation of the ende mic arsenic poisoning.METHODS Wistar rats were rando mly divided into arsenic 25,50 and 100mg·g-1 groups to be fed 164.67 pp marsenic conta minated grain at levels of 15%,30% and 60%,experi mental period was 90d,CJSOD intervention group was fed with feed contained arsenic 100mg·kg-1 for 90d,then CJSOD was ad ministered at 10mL·kg-1 (6d /week) for 45d.The urine arsenic contents,blood T-ly mphocyte subsets (CD3 +,CD4 +,CD8 + T cell),seru mimmunoglobulin (IgG,IgM,IgA)and complement (C3,C4)were analyzed.RESULTS Co mpared with the normal control group,As 50 and 100mg·kg-1 group had significantly lower CD3 +(46.21 ± 21 .62,31 .31 ±7.73),CD4 +(30.36 ±9.97,25.94 ±12.94)and the ratio of CD4 +/CD8 +(1 .12 ±0.41 , 1 .12 ±0.41 ).As 100mg·kg-1 group showed clearly high levels of C4(81 .18 ±13.23)(P <0.05). Co mpared with the As 100mg·kg-1 group,after intervention with CJSOD,the positive rate of CD3 +(54.06 ±25.77),CD4 +(42.50 ±17.01 ),and the ratio of CD4 +/CD8 +(1 .80 ±0.71 )were significantly increased,while the result of C4 (68.70 ±10.30)and the urine arsenic contents 〔253.82 (158.21 ~494. 11 )〕was significantly decreased(P <0.05).The levels of urine arsenic in rat and CD3 +,CD4 +and CD4 +/CD8 + were negatively correlated,while positive correlation existed between the level of urine arsenic and C4(P <0.01 ).CONCLUSION Arsenic inhibit the immune function in rats.Arsenic load levels were positively correlated with the degree of inhibition.CJSOD preparation can i mprove the immune function of arseniasis rats.