Smoking promotes the resorption of periodontal alveolar bone / 中国组织工程研究

ABSTRACT

BACKGROUND:Various studies confirm that smoking can contribute to the resorption of periodontal alveolar bone. OBJECTIVE:To observe the effect of smoking in alveolar bone resorption of periodontitis rat models. METHODS:A total of 24 rats were randomly divided into three groups. Normal grouprats were normal y fed without any other pre-treatment;Control groupexperimental periodontitis model was established using wire ligation method in rats;Experimental grouprat models were given passive smoking during experimental modeling period. Al rats were sacrificed after 8 weeks of modeling, periodontal tissue were removed. Hematoxylin-eosin staining was used for examining pathological changes in periodontal tissue, and immunohistochemical analysis was done for observing receptor activator of nuclear factor-κB ligand and osteoprotegrin expression. RESULTS AND CONCLUSION:After 8 weeks of modeling, expression of receptor activator of nuclear factor-κB ligand factor was significantly higher in alveolar bone of rats from experimental group in comparison to control group (P<0.05), whereas expression of osteoprotegerin in alveolar bone was significantly greater in rats from control group when compared to experimental group (P<0.05). This finding suggests that smoking can increase the expression of receptor activator of nuclear factor-κB ligand protein and reduce osteoprotegrin expression in periodontal rats, thus increasing the resorption of periodontal alveolar bone.