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Minocycline inhibits BV-2 cell activation by regulating P2X7 receptor / 中国病理生理杂志
Chinese Journal of Pathophysiology ; (12): 1066-1069, 2014.
Article de Zh | WPRIM | ID: wpr-451803
Bibliothèque responsable: WPRO
ABSTRACT
AIM: To explore the role of P2X7 receptor in inhibition of lipopolysaccharide (LPS)-stimulated BV-2 cell activation by minocycline .METHODS:BV-2 cells were divided into 5 groups:control group, LPS group, LPS+0.1 μmol/L Mino group, LPS+1 μmol/L Mino group and LPS+10 μmol/L Mino group.The expression of P2X7 re-ceptor was determined by real-time PCR and Western blotting .The levels of TNF-αand IL-1βin the microglia culture su-pernatants were measured by ELISA .The morphological changes of the cells were also observed .RESULTS: After ex-posed to LPS, the expression of P2X7 receptor increased in BV-2 cells at mRNA and protein levels .The concentrations of TNF-αand IL-1βin the microglia culture supernatants also increased .Meanwhile, 0.1~10μmol/L minocycline inhibited those changes in a dose-dependent manner .CONCLUSION:Minocycline inhibits the activation of microglia .The mecha-nism may be related to the P2X7 receptor.
Mots clés
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Chinese Journal of Pathophysiology Année: 2014 Type: Article
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Chinese Journal of Pathophysiology Année: 2014 Type: Article