Study on the protective effect of Cordyceps composite nutrient solution in hydrogen peroxide-induced PC12 cell injury / 中国生化药物杂志

ABSTRACT

Objective To study the protective effect of Cordyceps composite nutrient solution on hydrogen peroxide-induced PC12 cell injury.Methods P12 cells were divided into negative control group,model group,low-dose group(1%Cordyceps complex nutrient solution),middle dose group(3% Cordyceps complex nutrient solution)and high-dose group(5%Cordyceps complex nutrient solution).After 24 h cultured,except the negative control group,other groups were added H2 O2 and cultured for another 24h.Cell viability,LDH activity and NO were compared in each group. Results Survival rate in model group was(62.8 ±4.2)%,the survival rate of the negative control group was(100.0 ±1.7)%,the survival rate in the model group was significantly lower than that in negative control group(P<0.01 ),and cell viability increased significantly in composite nutrient solution group compared with model group(P<0.01).Cellular LDH levels and NO content in the model group and negative control group were significantly higher than that in negative control group(P<0.01),which decreased significantly when added cordyclps composite nutrient solution(P<0.01).Conclusion Cordyceps composite nutrient solution shows protective effect on hydrogen peroxide in PC12 cells.