The effects of IL-24 on the differentiation and function of osteoclasts / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 456-459, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-454194
ABSTRACT
Objective:
To study the effect of IL-24 on the differentiation and function of osteoclasts.Methods:
Mature osteoclasts were isolated from long bones of neonate rats.Optimal multiplicity of infection(MOI)of AdCMV-EGFP was determined.Then osteo-clasts and RAW264.7 cells were transfected with AdCMV-EGFP and AdCMV-IL-24 respectively.The function of osteoblasts was studied by the observation of bone resorption lacunae,the differentiation of RAW264.7 cells was evaluated by the expression of osteo-clast related genes examined with real-time PCR.Results:
Osteoclasts were TRAP positive with more than 2 neclei.MOI=400 was suitable for AdCMV-EGFP transfection.The resorption lacunae area in AdCMV-IL24 transfected cells was larger than that in AdCMV-EGFP transfected cells(P<0.05).Real-time PCR showed that under induced conditions,osteoclastic related genes NFATc1,CTSK and TRAP were changed in RAW264.7 cells transfected with AdCMV-IL-24.Conclusion:
IL-24 may promote the differentiation and function of osteoclasts.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Practical Stomatology
Année:
2014
Type:
Article
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