Effect of salidroside on activity of endothelial progenitor cells and phos-phoinositide 3-kinase/Akt signaling pathway / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 1192-1195, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-454724
ABSTRACT
AIM:
To investigate whether salidroside has influence on the activities of endothelial progenitor cells (EPCs) and its mechanism.METHODS:
Mononuclear cells from normal human peripheral blood were cultured in fi-bronectin coated flasks in endothelial progenitor medium .After 7 d, EPCs were characterized as adherent cells with acLDL-DiI uptaking and lectin binding by direct fluorescent staining .The proliferation and migration of EPCs were analyzed by MTT assay and Transwell chamber assay , respectively.The EPCs adhesion assay was performed by re-plating the cells on fibronectin-coated dishes , and then adherent cells were counted .NO and Akt protein were also detected .RESULTS:
Sali-droside promoted EPCs proliferative , migratory and adhesive capacities in a concentration dependent manner .Salidroside also increased NO secretion , and the level of phosphorylated Akt protein .However , the effects of salidroside on EPCs were inhibited by phosphoinositide 3-kinase inhibitor LY294002.CONCLUSION:
Salidroside regulates the activity of EPCs by phosphoinositide 3-kinase/Akt signaling pathway .
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Pathophysiology
Année:
2014
Type:
Article
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