Effect of Shenmai injection on Treg cells of idiopathic thrombocytopenic purpura / 中国基层医药

ABSTRACT

Objective To explore the effect of Shenmai injection on profile and function of CD4+CD2+5 Treg cells in ITP patients.Methods 78 ITP patients were selected and divided into the two groups, each group in 39 cases.The control group were given prednisone,while the observation group were given Shenmai injection additionally. CD4+CD2+5 FoxP3 +Treg cell counts were detected before and after treatment with flow cytometry,and the serum IL-2, IL-4 and IFN-γcontents were detected with ELISA kit.Results The effective rate of 56.41%and total effective rate of 92.31% in the observation group were higher than those of 30.77% and 71.79% in the control group (χ2 =4.222,3.933,all P<0.05).Furthermore,after the treatment,the CD2+5 FoxP3+Treg cell counts of 30.66%vs 24.10%and 7.49%vs 5.15%and serum IL-2,IL-4 and IFN-γcontents of (15.6 ±4.3)ng/mL vs (10.3 ±3.1)ng/mL, (9.5 ±2.7)ng/mL vs (13.9 ±3.4)ng/mL,(37.1 ±4.3)ng/mL vs (31.2 ±4.9)ng/mL in both the two groups increased(P<0.05);while all indexes′levels of the observation group were higher than those of the control group after treatment(χ2 =4.329,4.012,4.568,4.105,all P<0.05).Conclusion The defeat of CD4+CD2+5 Treg cells may be involved in the process of ITP,and Shenmai injection may be able to play a role in treatment through this link.