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Application of high resolution melting analysis in the rapid identification of functional class 2 inte-gron / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology ; (12): 849-851, 2014.
Article Dans Chinois | WPRIM | ID: wpr-458434
ABSTRACT
Objective To establish a high resolution melting based method for the rapid identifica-tion of functional class 2 integron.Methods Nighty-nine non-repetitive Proteus spp.strains positive for genes encoding class 2 integrase were isolated from August, 2011 to August, 2012.The genomic DNAs were extracted and used as templates to amplify 60 base pair fragments containing the mutated point in class 2 in-tegrase gene by PCR.The high resolution melting analysis was conducted to identify the functional class 2 in-tegrons that were further compared by sequence analysis.Results There were remarkable differences with the high resolution melting curves between the functional class 2 integrons and the ordinary class 2 integrons. The results of high resolution melting analysis were consistent with those by using sequence analysis.Con-clusion High resolution melting analysis could be used for the rapid and accurate identification of functional class 2 integron.

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Type d'étude: Etude diagnostique langue: Chinois Texte intégral: Chinese Journal of Microbiology and Immunology Année: 2014 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Type d'étude: Etude diagnostique langue: Chinois Texte intégral: Chinese Journal of Microbiology and Immunology Année: 2014 Type: Article