Determination of Chlorogenic Acid, Baicalin and Phillyrin in Yinhuanglian Oral Liquids by UPLC with Double-wavelength Switch / 中国药师

ABSTRACT

Objective:To establish a UPLC method for the content determination of chlorogenic acid, baicalin and phillyrin in Yinhuanglian oral liquids. Methods:An Agilent Eclipse Plus C18 column(100 mm × 2. 1mm,1. 8 μm)was used,the mobile phase was acetonitrile -0. 4% phosphoric acid with gradient elution,and the flow rate was 0. 2 ml·min-1 . The detection wavelength was set at 324 nm in the first 5 min for chlorogenic acid, and then changed to 277 nm for baicalin and phillyrin between 5 and 12 min . Results:The linear calibration curve of chlorogenic acid, baicalin and phillyrin was within the range of 39.4-355.0μg·ml-1(r=0.999 7), 90.0-810.0 μg·ml-1(r=0.999 9) and 9.4-84.6 μg·ml-1(r =0.999 5), respectively . The average recovery was 99.44%, 98. 82% and 98. 64%,respectively. Conclusion:The method is simple,reliable and accurate in the content determination of chloro-genic acid, baicalin and phillyrin in Yinhuanglian oral liquids.