Induced differentiation of adipose-derived stem cells / 中国组织工程研究

ABSTRACT

BACKGROUND:Adipose-derived stem cels are pluripotent stem cels developed from the mesoderm, which can differentiate into different lineages induced by specific growth factors and under certain environmental conditions. OBJECTIVE: To describe the induced differentiation and identification of adipose-derived stem cels in detail. METHODS:A computer-based search of Wanfang and PubMed databases was performed for relevant articles published from 2005 to 2014 using the keywords of “adipose derived stem cels, induced, differentiation” in Chinese and English, respectively. According to inclusion and exclusion criteria, 37 articles were selected in result analysis. RESULTS AND CONCLUSION:Adipose-derived stem cels can differentiate into chondrocytes under the induction of ascorbic acid, insulin, dexamethasone, and transforming growth factor β. Recipe for adipogenic induction medium consists of 3-isobutyl-1-methylxanthine (IBMX), insulin, dexamethasone, indomethacin; and commonly used inducers for osteogenic differentiation include dexamethasone or vitamin D3, ascorbic acid,β-glycerophosphate. Basic fibroblast growth factor, epidermal growth factor and vitamin B27 may be combined to induce the differentiation of adipose-derived stem cels into neurons. 5-Azacytidine acts as a commonly used factor for inducing the differentiation of adipose-derived stem cels into cardiomyocytes. Combination of vascular endothelial growth factor and basic fibroblast growth factor can induce adipose-derived stem cels to differentiate into vascular endothelial cels. With the rapid development of molecular biology and cel biology, research on the differentiation of adipose-derived stem cels wil be more in-depth. Based on the current observation of adipose-derived stem cel differentiation, internal molecular mechanism as wel as genes and proteins for regulation of adipose-stem cel plasticity should be explored in depth.