Hydrogen peroxide influences embryonic stem cell transplantation in the treatment of myocardial infarction / 中国组织工程研究

ABSTRACT

BACKGROUND:Specific mechanism of stem cel transplantation for treatment of myocardial infarction has been not very clear. OBJECTIVE:To investigate the effect of H2O2 METHODS: Twenty-four C57BL/6 mice, 9 weeks old, were randomized into myocardial infarction group, embryonic stem cels group, H-pretreated mouse embryonic stem cels in vitro for treatment of myocardial infarction. 2O2 pretreatment group, with eight mice in each group, which were respectively injected 150 μL PBS, 150 μL embryonic stem cels (1×106), and 150 μL H2O2-pretreated embryonic stem cels (1×106 RESULTS AND CONCLUSION:Compared with the myocardial infarction group, the left ventricular end-diastolic diameter and left ventricular end-systolic inner diameter were significantly decreased in the embryonic stem cels ) at 1 week after modeling. At 3 weeks after transplantation, left ventricular end-diastolic diameter, left ventricular end-systolic inner diameter and left ventricular ejection fraction of mice in each group were measured by ultrasound. Myocardial colagen content was detected by Sirius red staining and the average colagen volume fraction was calculated. group and H2O2 pretreatment group, while the left ventricular ejection fraction was significantly increased (P < 0.05). Heart weight/body weight, left ventricular weight/body weight, and average colagen volume fraction were significantly lower in the embryonic stem cels group and H2O2 pretreatment group than the myocardial infarction group (P < 0.05). Therefore,in vitro H2O2 pretreatment enhances reduce the post-myocardial infarction myocardial fibrosis and greatly improve heart function.