MicroRNA-21 for regulation of TLR4 in Hela cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 220-224, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-462331
ABSTRACT
BACKGROUND:
MicroRNAs (miRNA) through regulating specific target gene mRNA expression play important roles in different processes of diseases.OBJECTIVE:
To study the interaction of miRNA-21 with its target gene TLR4 in Hela cels.METHODS:
The candidate target gene of miRNA-21 was determined according to miRNA analysis databases. The constructed recombinant adenovirus vector carrying pri-miRNA-21 gene was used, which could package and amplify viruses to transfect Hela cels. Then, the expression of fluorescent proteins was detected. Forty-eight hours after transfection of miRNA-21 or control, extracted proteins were used for detection of TLR4 protein using western blot assay. RESULTS ANDCONCLUSION:
Recombinant adenoviruses pAd/pri-miRNA-21 and pAd/neg at 100 MOI could successfuly infect Hela cels. Bioinformatic analysis suggested several possible binding sites between miRNA-21 and TLR4. The experimental results showed that miRNA-21 down-regulated TLR4 at protein levels, indicating that miRNA-21 can interfere with the expression of TLR4 target gene.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Tissue Engineering Research
Année:
2015
Type:
Article
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