The relationship between human adiponectin gene polymorphism of Guangxi Zhuang ethnic group and gestational diabetes mellitus / 中国医师杂志

ABSTRACT

Objective To investigate the association of single nuchotide polymorphism (SNP) + 45 T/G and + 276 G/T in the adiponectin gene with type 2 diabetes in Guangxi Zhuang pregnant women.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the distributions of allele and genotype frequencies of SNP + 45T/G polymorphism and SNP + 276 G/T polymorphism in adiponect in gene in 98 type 2 diabetic patients and 135 normal control subjects.Results The TG,GG genotype frequencies of the SNP +45T/G were higher in gestational diabetes mellitus (GDM) than those in controls (P ＜ 0.05).The G-allele frequency was significantly higher than those in controls (P ＜ 0.05).The distribution of genotype and allele frequencies of the SNP + 276 G/T polymorphism was not different in diabetes and control groups (P ＞ 0.05).Women carrying the TG and GG allele of SNP +45T/G showed higher TG (P ＜0.05),and lower APN levels than those with TT genotype (P ＜0.05).Conclusions G allele of SNP +45T/G was related to GDM possibly.T→G variation might involve in the occurrence of GDM by reducing serum diponectin levels.Monitoring this site in pregnant women could predict the risk of GDM.