Molecular Variation and Distribution of Anopheles fluviatilis (Diptera: Culicidae) Complex in Iran
The Korean Journal of Parasitology
;
: 231-236, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-46697
ABSTRACT
Anopheles fluviatilis James (Diptera Culicidae) is one of the known malaria vectors in south and southeastern Iran. Earlier ITS2 sequences analysis of specimens from Iran demonstrated only a single genotype that was identical to species Y in India, which is also the same as species T. We identified 2 haplotypes in the An. fluviatilis populations of Iran based on differences in nucleotide sequences of D3 domain of the 28S locus of ribosomal DNA (rDNA). Comparison of sequence data from 44 Iranian specimens with those publicly available in the Genbank database showed that all of the 28S-D3 sequences from Kazeroun and Khesht regions in Fars Province were identical to the database entry representing species U in India. In other regions, all the individuals showed heterozygosity at the single nucleotide position, which identifies species U and T. It is argued that the 2 species may co-occur in some regions and hybridize; however, the heterozygosity in the 28S-D3 locus was not reflected in ITS2 sequences and this locus for all individuals was identical to species T. This study shows that in a newly diverged species, like members of An. fluviatilis complex, a single molecular marker may not be sufficiently discriminatory to identify all the taxa over a vast geographical area. In addition, other molecular markers may provide more reliable information for species discrimination.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Variation génétique
/
Données de séquences moléculaires
/
ARN ribosomique 28S
/
Séquence nucléotidique
/
Alignement de séquences
/
Analyse de séquence d'ADN
/
Espaceur de l'ADN ribosomique
/
Vecteurs insectes
/
Iran
Limites du sujet:
Animaux
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2010
Type:
Article
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