Trueness Assessment for Serum Glucose Measurement Using Commercial Systems through the Preparation of Commutable Reference Materials
Annals of Laboratory Medicine
;
: 243-249, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-47756
ABSTRACT
BACKGROUND:
Commutable reference materials (RMs) are suitable for end-users for evaluating the metrological traceability of values obtained using routine measurement systems. We assessed the performance of 6 routine measurement systems with validated secondary RMs.METHODS:
We tested the homogeneity, stability, and commutability of 5 minimally processed human serum pools according to the standard guidelines. The serum pools were assigned values as per the reference procedure of the United States Centers for Disease Control and were used to evaluate the trueness of results from 6 commercial measurement systems based on enzymaticmethods:
3 glucose oxidase (GOD) and 3 hexokinase (HK) methods.RESULTS:
The prepared RMs were validated to be sufficiently homogenous, stable, and commutable with the patient samples. Method bias varied for different systems GOD01, -0.17 to 2.88%; GOD02, 1.66 to 4.58%; GOD03, -0.17 to 3.14%; HK01, -3.48 to -0.85%; HK02, -3.83 to -0.11%, and HK03, -1.82 to -0.27%.CONCLUSIONS:
We observed that the prepared serum glucose RMs were qualified for trueness assessment. Most of the measurement systems met the minimal quality specifications.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Trousses de réactifs pour diagnostic
/
Normes de référence
/
Analyse chimique du sang
/
Glycémie
/
Analyse de régression
/
Glucose oxidase
/
Hexokinase
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Annals of Laboratory Medicine
Année:
2012
Type:
Article
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