Occlusal stimulation regulates bone remodeling during bone defect repair using collagen substitutes：a morphological analysis / 中国组织工程研究

ABSTRACT

BACKGROUND:Occlusal stimulation is essential for mandible function and remodeling, but there is stil a lack of clear understanding about the effect of occlusal stimulation on the bone remodeling in the process of bone defect repair using bone grafts. OBJECTIVE:To analyze the possible regulative effect of occlusal stimulation on bone remodeling in the process of bone defect repair using colagen substitutes. METHODS:Standard models of bone defects were respectively established in left mandible and parietal bone area of adult Sprague-Dawley rats. Then the bone defects area were filed with colagen and bone meal. The differences of two bone defects areas were observed by X-ray, hematoxylin-eosin staining, Gomori staining, tartrate-resistant acid phosphatase staining and bone morphogenetic protein 2 immunohistochemical staining at the 12th week after operation. RESULTS AND CONCLUSION: New bone formation was visible in the bone defect regions of the mandible and parietal bone. The amount of lamelar bone formation and the degree of mineralization of the new bone were significantly increased in the parietal bone defect compared with the mandibular bone defect area, indicating the bone remodeling in the parietal bone defect area was better than that in the mandible bone defect area. The integral absorbance values of tartrate-resistant acid phosphatase and bone morphogenetic protein 2 in the parietal bone defect area were lower than those in the mandibular bone defect area, indicating that the viabilities of osteoblasts and osteoclasts in the parietal bone defect area were lower than those in the mandible bone defect area. These results demonstrate that occlusal stimulation may delay the bone remodeling during the repair of mandibular bone defects by regulating bone mineralization and maturation.