The application of multicolor probe melting curve analysis for the prenatal diagnosis ofβthalassemia / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
;
(12): 192-196, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-487487
ABSTRACT
Objective To establish a method of multicolor melting curve analysis for the prenatal diagnosis ofβthalassemia.Methods Methodology establishment.A total of 95 cases, including 9 fetal villi samples(10-13 weeks)and 86 amniotic fluid samples(18-24 weeks)were collected by Center for Prenatal Diagnosis of Shenzhen Maternity and Child Healthcare Hospital between January 2014 and December 2014.A double-blind test was done to detect the mutations of beta globin gene by means of reverse dot ( RDB) blot and multicolor melting curve analysis ( MMCA).The consistency of the two methods is compared.Results The results of 93 cases detected by MMCA and RDB are completely consistent.The results of the 2 cases detected by MMCA after correction are the same as the results detected by RDB.Finally, the coincidence rate of the result was 100%.Conclusion MMCA can be applied to the prenatal diagnosis ofβthalassemia as an effective supplement to RDB.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Essai clinique contrôlé
/
Etude diagnostique
langue:
Chinois
Texte intégral:
Chinese Journal of Laboratory Medicine
Année:
2016
Type:
Article
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