Simultaneous Content Determination of Quercetin and Kaempferol in Penthorum Chinense Pursh by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish an HPLC method for the simultaneous content determination of quercetin and kaempferol in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Kromasil C18 (250 mm× 4.0 mm, 5.0 μm) with a mixture of methanol-0.1% phosphate (50:50) as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1.0 mL/min. The column temperature was set at room temperature. Results The quercetin and kaempferol showed good linearity in the range of 0.105 6–2.112 0 μg and 0.023 6–0.472 μg respectively. The average recovery rates of quercetin and kaempferol were 98.46% (RSD=2.29%) and 98.17%(RSD=1.99%) respectively. Conclusion The method is accurate and sensitive, which can be used for the content determination of quercetin and kaempferol in Penthorum Chinense Pursh.