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A New Prenatal Diagnosis of Fetal Cells Isolation from Maternal Peripheral Blood -Using Comparative Genomic Hybridization by Microdissection / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology ; : 213-219, 2002.
Article Dans Coréen | WPRIM | ID: wpr-48885
ABSTRACT

OBJECTIVE:

The objective of this study was to determine the clinical use of CGH (comparative genomic hybridization) for detection of fetal aneuploidy from fetal cells (nucleated red blood cells, nRBCs) isolated from maternal peripheral blood.

METHODS:

Maternal peripheral venous blood sample was collected and treated by heparin. Triple density gradient centrifugation, and MACS (magnetic activated cell sorting) using CD45 and CD 71 were used to isolated the fetal nRBCs. With microdissection, DOP (degenerate oligonucleotide primed)-PCR (polymerase chain reaction), and nick translation, CGH was performed.

RESULTS:

Fetal nRBCs were successfully isolated from maternal peripheral blood. After microdissection of fetal nRBCs, DOP-PCR. and nick translation, DNA size was suitable for hybridization. In CGH analysis, we can confirm normal female and trisomy 21 male fetus.

CONCLUSION:

Prenatal diagnosis from fetal cells in maternal peripheral blood by comparative genomic hybridization shows clinical promise in terms of speed, accuracy, and non-invasiveness. To enable widespread use of this method, further studies involving many cases are warrented.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Diagnostic prénatal / ADN / Héparine / Centrifugation en gradient de densité / Syndrome de Down / Microdissection / Érythrocytes / Hybridation génomique comparative / Foetus / Aneuploïdie Type d'étude: Etude diagnostique Limites du sujet: Femelle / Humains / Mâle langue: Coréen Texte intégral: Korean Journal of Obstetrics and Gynecology Année: 2002 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Diagnostic prénatal / ADN / Héparine / Centrifugation en gradient de densité / Syndrome de Down / Microdissection / Érythrocytes / Hybridation génomique comparative / Foetus / Aneuploïdie Type d'étude: Etude diagnostique Limites du sujet: Femelle / Humains / Mâle langue: Coréen Texte intégral: Korean Journal of Obstetrics and Gynecology Année: 2002 Type: Article