The expression of 5-hydroxytryptamine receptor 2B in gastric cancer and its clinical significance / 中华普通外科杂志

ABSTRACT

Objective To explore the expression and distribution characteristics of 5-hydroxytryptamine receptor 2B (5-HT2BR) in tumor tissue,blood and gastric juice of patients with gastric cancer.Methods From 2006 to 2013 gastric cancer tissue paraffin specimens were collectec from 30 stomach cancer patients,gastric ulcer paraffin specimens from 10 patients,and gastric juice,blood samples and fresh tumor tissues were collected from gastric cancer patients undergoing surgery.Corresponding samples were collected in 15 cases of chronic superficial gastritis while receiving gastroscopy.By using ELISA and PV-9000 immunohistochemistry 5-HT2BR expression was determined.Results There was no positive expression of 5-HT2BR in benign gastric tissues (n =15).The positive expression rate of 5-HT2BR between gastric cancer and gastric benign lesions has significant difference (P＜0.01).Integral optical density value and positive area of 5-HT2BR in low differentiation tissues of gastric cancer were higher than high and medium differentiation tissues of gastric cancer (P ＜ 0.01).There was no statistically significant difference among 5 HT2BR and constituent ratio of patients at tumor stage,differentiated degree,histological type,infiltration depth and lymph node metastasis (all P ＞ 0.05).The differences of 5-HT2BR expression in gastric juice and blood between gastric cancer patients and chronic gastritis patients are statistically significant(P ＜ 0.01).Conclusions 5-HT2BR are highly expressioned in blood,gastric juice and tumor tissues of patients with gastric cancer in a differentiation related manner.