Effects of propofol on the mitochondria ATP-sensitive potassium channel that contains Kir6.2 and SUR1 subunits in rats with cerebral ischemia-reperfusion injury / 临床麻醉学杂志

ABSTRACT

Objective To evaluate the effects of propofol on the mitochondria ATP-sensitive potassium channel that contains Kir6.2 and SUR1 subunits in rats with cerebral ischemia-reperfusion injury.Methods Twenty-four male Sprague-Dawley rats,weighing 280-320g,were randomly divided into 3 groups (n = 8)sham operation group(group S),cerebral ischemia-reperfusion group (group IR)and propofol group (group P).Transient focal cerebral ischaemia and reperfusion injury was in-duced by middle cerebral artery occlusion model in group P and group IR.Rats in group P were injec-ted 50 mg/kg propofol 2hr prior to ischemia.In groups S and IR,1 ml of normal saline was injected instead.The neurological scores were evaluated and brain infarction size was measured using TTC-staining.The mRNA expression levels of Kir6.2 and SUR1 were analyzed by quantitative polymerase chain reaction,while the protein levels of Kir6.2 and SUR1 were determined by western blot analysis. Results Compared with group S,neurological scores and infarction size was increased in group IR and group P,and the mRNA and protein levels of Kir6.2 in the group IR were down-regulated(P <0.05).Compared with group IR,neurological scores and infarction size decreased and the mRNA and protein levels of Kir6.2 were up-regulated in group P(P < 0.05 );the expressions of SUR1 in the three groups showed no significant difference.Conclusion Propofol could attenuate cerebral ischemia and reperfusion injury through up-regulating Kir6.2 expression in rats.