Culture of Dendritic Cell from Normal Peripheral Blood Monocyte and Its Anti-tumor Immune Activity When Pulsed by Renal Cell Carcinoma Cell Line: In vitro Study / 대한비뇨기과학회지
Korean Journal of Urology
;
: 795-801, 2002.
Article
Dans Coréen
| WPRIM
| ID: wpr-49234
ABSTRACT
PURPOSE:
Dendritic cells (DC) play a crucial role in the initiation of primary immune response and are known as an excellent adjuvant for anti-cancer immunity. In this study, we tried to obtain substantial numbers of DC from peripheral blood of normal volunteers. We also investigated the anti-tumor immune response of DC pulsed by renal cell carcinoma cell line A498 in vitro. MATERIALS ANDMETHODS:
DC were generated by culturing plastic adherent mononuclear cells from the peripheral blood in the presence of granulocyte-macrophage colony- stimulating factor and interleukin-4. Immature DC were cocultured with T-cells and pulsed by A498. MTT analysis was performed using the medium in which A498 only was cultured as control. Our experiments were analyzed by means of a commercial IL-12 p70 ELISA (Quantikine; R & D Systems, Minneapolis, MN). The capture antibodies used in both tests specifically recognize the p70 heterodimer, but not the free p40 chains. Detection limits were 30pg/ml of IL-12.RESULTS:
We could obtain 1.5-2.0x106 DC with phenotype typical of mature DC (CD14-, CD80+, and CD83+) from the normal peripheral blood. On T-cell proliferation assay, the number of T-cells increased in proportion to that of DC and when DC were pulsed by A498, the same phenomenon could be observed. DC and T-cell media with A498 tumor lysate showed more production of IL-12 on IL-12 p70 ELISA than the media without A498 tumor lysate.CONCLUSIONS:
We could successfully obtain mature DC from the peripheral blood. The data revealed indirectly that DC treated with tumor lysate enhance immune activity and thereby increase the anti-cancer effect of T-cells. Further investigations including in-vivo study are necessary to realize the effect of immunotherapy using DC against metastatic renal cell carcinoma.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phénotype
/
Matières plastiques
/
Cellules dendritiques
/
Test ELISA
/
Néphrocarcinome
/
Monocytes
/
Lymphocytes T
/
Lignée cellulaire
/
Interleukine-4
/
Interleukine-12
langue:
Coréen
Texte intégral:
Korean Journal of Urology
Année:
2002
Type:
Article
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