Effect of Protamine Coated PLA Nanocapsule on the Immune Function of Murine BMDCs In Vitro / 华中科技大学学报(医学版)
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
; (6): 298-301, 2016.
Article
de Zh
| WPRIM
| ID: wpr-494315
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To investigate the effect of protamine(PS)coated polylactic acid(PLA)nanocapsule on the immune response of murine bone marrow-derived dendritic cells(BMDCs).Methods PLA nanocapsules were prepared by W1/O/W2 method.Protamine was used to modify the surface of PLA nanocapsule that encapsulated ovalbumin(OVA),a model anti-gen.The prepared OVA/PLA/PS nanocapsules were used to stimulate BMDCs.Flow cytometry was used to analyze the ex-pression of surface molecules CD80,CD83,CD86,MHCⅠ,and MHC Ⅱ,and the uptake of nanocapsule.The levels of IL-12 p70 secreted by BMDCs were detected by ELISA.Results OVA/PLA/PS nanocapsules could significantly up-regulate the expres-sion of CD80,CD83,MHCⅠ,and MHC Ⅱ in BMDCs,and increase the secretion of IL-12 p70 by BMDCs.Furthermore,OVA/PLA/PS nanocapsules could enhance the uptake efficiency of OVA by BMDCs.Conclusion OVA/PLA/PS nanocapsule can en-hance the immune response of BMDCs,and may become a good drug delivery carrier.
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WPRIM
langue:
Zh
Texte intégral:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
Année:
2016
Type:
Article