Expression and significance of miR-146a and LIN52 in advanced gastric cancer / 肿瘤研究与临床
Cancer Research and Clinic
; (6): 388-393, 2016.
Article
de Zh
| WPRIM
| ID: wpr-497228
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To explore the expression of miR-146a and its target gene LIN52 in advanced gastric cancer and their potential impact on clinical prognosis.Methods Total RNAs were extracted from 93gastric cancer tissues and their corresponding adjacent non-tumor tissues to quantify the relative expression of miR-146a by using real-time quantitative PCR (RT-qPCR).Expression of LIN52 was detected in tumors and normal tissues by immunohistochemistry.Correlation analysis was assessed between the expression of miR-146a and LIN52 and clinicopathological parameters,including clinical diagnostic specificity,tumor TNM staging,lymph node metastasis,differentiation grade,curative effect and prognosis of gastric cancer.Results The expression of miR-146a in gastric carcinoma was negatively correlated with lymph node metastasis (P <0.05).The expression of miR-146a had a significant correlation with the prognosis of the patients (P < 0.01).The patients with high expression of miR-146a had higher survival rate (P < 0.05),but the patients with high expression of LIN52 had lower survival rate (P < 0.05).The receiver operating characteristic curve regression analysis showed that sensitivity and specificity of miR-146a were 94.1% and 61.5 % to diagnose gastric cancer.Conclusions As a tumor suppressor gene in gastric cancer,miR-146a has significantly negative correlation with LIN52.High expression of miR-146a in the gastric cancer tissue might be associated with improved treatment efficacy of chemotherapy,suggesting that miR-146a may be a molecular marker for the diagnosis and prognosis of gastric cancer.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Prognostic_studies
langue:
Zh
Texte intégral:
Cancer Research and Clinic
Année:
2016
Type:
Article