PCR-based identification of methicillin-resistant Staphylococcus aureus strains and their antibiotic resistance profiles
Asian Pacific Journal of Tropical Biomedicine
;
(12): 293-297, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-500619
ABSTRACT
Objective:
To evaluated the PCR for mecA gene compared with the conventional oxacillin disk diffusion method for methicillin-resistant Staphylococcus aureus (S. aureus) identification.Methods:
A total of 292 S. aureus strains were isolated from various clinical specimens obtained from hospitalized patients. Susceptibility test to several antimicrobial agents was performed by disk diffusion agar according to Clinical and Laboratory Standards Institute guidelines. The PCR amplification of the mecA gene was carried out in all the clinical isolates.Results:
activity and vancomycin was the most effective. The rate of methicillin-resistant S. aureus prevalence determined by oxacillin disk diffusion method was 47.6%; whereas, 45.1% of S. aureus isolates were mecA- positive in the PCR assay. Among antibiotics used in our study, penicillin showed the least anti-staphylococcalConclusions:
This study is suggestive that the PCR for detection of mecA gene is a fast, accurate and valuable diagnostic tool, particularly in hospitals in areas where methicillin-resistant S. aureus is endemic.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
/
Guide de pratique
langue:
Chinois
Texte intégral:
Asian Pacific Journal of Tropical Biomedicine
Année:
2014
Type:
Article
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