The Effects of Scorpion’s Proteins with Different Molecular Weights on Angiogenesis of the Transgenic Ze-brafish / 中国药房
China Pharmacy
;
(12): 3490-3492, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-501052
ABSTRACT
OBJECTIVE:
To study the effects of scorpion’s proteins with different molecular weights on angiogenesis of the transgenic zebrafish.METHODS:
The vascular fluorescence transgenic zebrafish models were established. Scorpion’s proteins were separated by ultrafiltration and ion exchange chromatography to obtain the scorpion protein fractions with different molecular weights (3-10 ku,>10-50 ku and>50 ku). The embryos of transgenic zebrafishes were cultured in the above 10,100 and 500 μg/ml scor-pion’s proteins. Intersegmental vessels of the transgenic zebrafishes were counted under the fluorescence microscope to optimize the most suitable scorpion’s protein molecular weight. The vessels were counted again with >50 ku scorpion protein component 1 and 2,so as to select suitable component.RESULTS:
The >50 ku scorpion’s protein fraction component 1 with the mass concentration of 500 μg/ml had the highest inhibitory activity for the angiogenesis of the transgenic zebrafish,with inhibitory rate of 92.59%.CONCLUSIONS:
Scorpion’s protein and its fractions have the activity of angiogenesis inhibition,which may be one of anti-cancer mechanisms of scorpion.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
China Pharmacy
Année:
2015
Type:
Article
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