Study on the Content Dynamic Changes of Caffeic Acid,Vicenin-2 and Isoschaftoside in Rabdosia lophanthoi-des / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for simultaneous determination of caffeic acid,vicenin-2 and isoschaftoside in Rabdosia lophanthoides to assess its optimal harvest period. METHODS:HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-0.1% phosphoric acid solution (gradient elution) at a flow rate of 0.8 ml/min ,detection wave-length was 334 nm ,column temperature was 25 ℃ and volume injection was 15 μl. RESULTS:The linear range was 0.23-5.68 μg/ml for caffeic acid(r=0.999 9),0.31-7.76 μg/ml for vicenin-2(r=0.999 8)and 0.45-11.30 μg/ml for isoschaftoside(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%,average recoveries were 95.1%-98.9%(RSD=1.8%, n=6),97.4%-101.3%(RSD=1.7%,n=6) and 95.5%-98.8%(RSD=1.4%,n=6),respectively. The contents of above-men-tioned 3 ingredients of R. lophanthoides were the highest harvested in Apr. to May and Jul. to Aug. in a year. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the simultaneous determination of caffeic acid,vicenin-2 and isoschaftoside in R. lophanthoides. The optimal harvest period is Apr. to May and Jul. to Aug. in a year.