Content Determination of Dextran70 in Compound Dextran70 Eye Drop by HPLC-RID / 中国药房
China Pharmacy
;
(12): 5126-5128, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-501333
ABSTRACT
OBJECTIVE:
To establish a method for determination of dextran70 in Compound dextran70 eye drop.METHODS:
HPLC-RID was performed on the column of Phenomenex PolySep-GFC-P 4000 with the mobile phase of 0.7% Na2SO4 solution. The flow rate was 0.5 ml/min,column temperature was 35 ℃ and injection volume was 50 μl,and the inner temperature of refrac-tive index detector was 40 ℃.RESULTS:
The linear range of dextran70 was 0.1-5.0 mg/ml(r=0.999 5);RSDs of precision,sta-bility and reproducibility tests were lower than 2%;average recovery was 97.45%-101.76%(RSD=0.57%,n=9). CONCLU-SIONSThe method is simple,fast and reliable with high separation and short analysis time,and can be used for the content deter-mination of dextran70 in Compound dextran70 eye drop.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
China Pharmacy
Année:
2015
Type:
Article
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