Optimization of Comprehensive Extraction Process of Crude Polysaccharides and Total Saponins from Fu-ling Kugua Capsule by Response Surface Methodology / 中国药房

ABSTRACT

OBJECTIVE:To optimize the comprehensive extraction technology of crude polysaccharide and total saponins from Fuling kugua capsule. METHODS:Based on single factor test,using liquid ratio,extraction time and extraction times as response factors,the parameters of extraction technology of crude polysaccharides and total saponins from Fuling kugua capsule were opti-mized by using Box-Behnken design of response-surface method. RESULTS:The optimal extraction technology was as follows as liquid ratio of 112,extraction time for 1.4 h,extraction times for 2 times. Under this technology,the yield of crude polysaccha-ride was 14.44%(RSD=0.14%,n=3),and that of total saponins was 1.33%(RSD=0.75%,n=3). They were close to predict-ed value 14.69% and 1.36%,and the deviation were 1.70% and 2.21%. CONCLUSIONS:Measured value is close to predicted value,which indicates comprehensive extraction parameters optimized by response-surface methodology can be sued for the extrac-tion of crude polysaccharide and total saponins from Fuling kugua capsule.