Content Determination of Glabridin in Clycyrrhizae Radix et Rhizoma from Different Regions by HPLC / 中国药房
China Pharmacy
;
(12): 824-826, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-504302
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of glabridin in Clycyrrhizae Radix et Rhizoma from different regions.METHODS:
HPLC was performed on the column of Inertsil ODS-SP with mobile phase of acetoni-trile-0.05%phosphoric acid(gradient elution)at a flow rate of 0.8 ml/min,detection wavelength was 280 nm,the column tempera-ture was 35 ℃,and the injection volume was 20 μl.RESULTS:
The linear range of glabridin was 0.906-18.12 μg/ml(r=0.999 7), RSDs of precision,stability and reproducibility tests were lower than 3%;recovery was 98.73%-101.90%(RSD=1.25%,n=6). There were obvious differences among the glabridin contents in G. uralensis from different regions.CONCLUSIONS:
The method is simple and accurate with high precision,and can be used for the content determination of glabridin in Clycyrrhizae Radix et Rhi-zoma.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
China Pharmacy
Année:
2016
Type:
Article
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