Effects of Sodium Butyrate on Spatial Learning and Memory Ability in Chronic Stress Model Rats / 中国药房

ABSTRACT

OBJECTIVE:To study the effects of sodium butyrate on spatial learning and memory ability in chronic stress model rats. METHODS:SD rats were randomly divided into control group(normal saline),model group(normal saline)and sodium bu-tyrate low-dose,medium-dose and high-dose groups (50,100,200 mg/kg) with 14 rats in each group. Model group and sodium butyrate groups received plantar stimulation by electric shock for 21 d,once a day,2 h each time,to induce chronic stress model. They were given relevant medicine ip 0.5 h before stimulation. Morris water maze test was adopted to test spatial learning and mem-ory ability of rats (using escape latency as index,for consecutive 5 d). Western blot assay was used to detect the expression of acH3,acH4,cAMP,p-CREB and BDNF in hippocampus. RESULTS:Compared with control group,escape latency of model group and sodium butyrate groups prolonged significantly on the second day (P<0.05),and the expression of acH3,acH4, p-CREB and BDNF decreased(P<0.05). Compared with model group,escape latency of sodium butyrate groups shortened,espe-cially in high-dose group(there was statistical significance on the second day of test)(P<0.05);the expression of acH3,acH4, p-CREB,and BDNF enhanced (P<0.05). CONCLUSIONS:Sodium butyrate can improve chronic stress-induced the decrease of spatial learning and memory ability,the mechanism of which may be recovering histone acetylation level of chronic stress rat hippo-campus,and increasing cognitive related proteins such as p-CREB and BDNF.