Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
The Korean Journal of Parasitology
;
: 375-380, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-50648
ABSTRACT
Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Test ELISA
/
Dosage immunologique
/
Tests sérologiques
/
Sensibilité et spécificité
/
Angiostrongylus cantonensis
/
Diagnostic
/
Limite de détection
/
Angiostrongylus
/
Méthodes
/
Anticorps monoclonaux
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Humains
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2016
Type:
Article
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